Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1979-04-02
1981-03-10
Roberts, Elbert L.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
26023957, 2603131, 560 20, 562433, 546 44, 544299, 548361, C07J 7100
Patent
active
042553296
ABSTRACT:
A novel sensitive method for determining qualitatively and quantitatively the presence of a wide variety of physiologically active organic compounds (ligand) and their receptors is provided. The method employs a reagent which involves bonding a compound having structural similarity to the compound to be determined (ligand analog) to a fluorescing compound. The unknown compound is referred to as a ligand, the conjugate of the structurally similar compound and fluorescer is referred to as ligand analog-fluorescer, and compounds which recognize a specific structure and bind to such structure as referred to as receptors and are normally antibodies.
The fluorescer which is chosen will have either a change is quantum yield or a change in its emission and/or absorption spectra or all of them, when bound to antibody, as compared to being unbound. For the purposes of the assay, all that is required is that there be a change in the emission intensity at some wavelength or band of wavelengths.
The rate at which fluorescer antibody binds to the fluorescer portion of the ligand analog-fluorescer or the amount of fluorescer antibody bound to the fluorescer portion of the ligand analog-fluorescer at equilibrium will be related to the amount of ligand antibody bound to the ligand analog portion of the ligand analog-fluorescer. Therefore, by combining antibodies to both ligand and fluorescer, with ligand analog-fluorescer and an unknown, one can determine the amount of ligand present in the unknown by relating the emission intensity at a particular wavelength or band of wavelengths to standards.
Alternatively, by combining an unknown containing antiligand with fluorescer antibody and ligand analog-fluorescer, one can determine the amount of receptor in the unknown.
REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3855208 (1974-12-01), Rutner et al.
patent: 3981982 (1976-09-01), Oslapas et al.
Roberts Elbert L.
Rowland Bertram I.
Syva Company
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