Double mutants of bacteriorhodopsin

Details Double mutants of bacteriorhodopsin Double mutants of bacteriorhodopsin

1991-11-14

1994-03-01

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353171, 4353201, 530350, C12N 121, C12N 1574, C07K 1300

Patent

active

052906990

ABSTRACT:
Double mutants of bacteriorhodopsin are mutated in the amino acid positions 85 and 96 and are expressed in Halobacteria. The mutants have an altered absorption maximum of their ground state and of their intermediate with the longest life. These mutants pump anions in place of protons.

REFERENCES:
Mogi et al., "Aspartic Acid Substitutions affect proton translocation by teriorhodopsin," PNAS USA, 85:4148-4152. (Jun. 1988).
Butt et al., "Aspartic Acids 96 and 85 play a central role in the function of bacteriorhodopsin as a proton pump," EMBO J., 8(6):1657-1663. (1989).
Ni et al, "An efficient system for the synthesis of bacteriorhodopsin in Halobacterium halobium," Gene 90:169-172. (1990).
Oesterhelt et al., "Two pumps, one principle: light driven ion transport in Halobacteria," TIBS 14:57-61. (Feb. 1989).
D. Oesterhelt and Stoechenius, Methods Enzymol. 31, Biomembranes (1974), pp. 667-678, "Isolation of the Cell Membrane of Halobacterium halobium and Its Fractionation into Red and Purple Membrane".
Biosystems 19 (1986), pp. 223-236, "The Bacteriorhodopsin Model Membrane System as a Prototype Molecular Computing Element".
Soppa et al., J. Biol. Chem., 264, 22 (1989), pp. 13049-13056, "Bacteriorhodopsin Mutants of Halobacterium sp. GRB".
Subramaniam et al., Proc. Natl. Acad. Sci. USA, 87 (1990), p. 1013-1017, "Protonation state of Asp (Glu)-85 regulates the purple-to-blue transition in bacteriorhodopsin mutants Arg-82.fwdarw.Ala and Asp-85.fwdarw.Glu: The blue form is inactive in proton translocation".
Dassarma et al., Proc. Natl. Acad. Sci. USA, 81 (1984), p. 125-129, "Bacterio-opsin mRNA in wild-type and bacterio-opsin-deficient Halobacterium halobium strains".
Cline et al., J. Bacteriol. 171 (1989), pp. 4987-4991, "Transformation of the Archaebacterium Halobacterium volcanii with Genomic DNA".
Cline et al., Can. J. Microbiol 35 (198), pp. 148-152, "Transformation methods for halophilic archaebacteria".
Hackett and Dassarma, Can. J. Microbiol 35 (1989), pp. 86-91, "Characterization of the small endogenous plasmid of Halobacterium strain SB3 and its use in transformation of H. halobium".
Lam and Doolittle, Proc. Natl. Acad. Sci. USA, 86 (1989), pp. 5478-5482, "Shuttle vectors for the archaebacterium Halobacterium volcanii".
Stanssens et al., Nucleic Acid. Res. 17 (1989), pp. 4441-4454, "Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers".
Bamberg et al., Biochim. Biophys. Acta 773 (1984), pp. 53-60, "Reconstitution of the light-driven electrogenic ion pump halorhodopsin in black lipid membranes".

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