Double-labeled enzymeimmunoassay methods

Details Double-labeled enzymeimmunoassay methods Double-labeled enzymeimmunoassay methods

1986-01-22

1989-02-28

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435810, 436501, 436518, G01N 3353, G01N 33543, G01N 33566, C12Q 100

Patent

active

048085219

ABSTRACT:
The present invention provides a method of performing an immunoassay of a ligand in a liquid sample wherein two independently measureable enzyme labels are separately conjugated to two or more components or populations of components of the assay system and, after completion of the complexing reaction, substantially all of the first enzyme label and a proportion of the second enzyme label are removed from the assay mixture, the proportion of the second label removed being related to the amount of the said ligand and the assay determined from a measurement of said proportion of the second label being normalised by comparison with a measurement of the total first label removed.
In this way, manual pipetting errors and other assay procedural errors can be corrected for.

REFERENCES:
patent: 4540659 (1985-09-01), Litman et al.
patent: 4659678 (1987-04-01), Forrest et al.

Affiliated with

Also associated with

Rating

Double-labeled enzymeimmunoassay methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Double-labeled enzymeimmunoassay methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Double-labeled enzymeimmunoassay methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1366596