Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-07-17
2007-07-17
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100
Reexamination Certificate
active
10768729
ABSTRACT:
This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
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Chen Yi-Ju
Leamon John H.
Lohman Kenton L.
Ronan Michael T.
Rothberg Jonathan M.
454 Life Sciences Corporation
Elrifi, Esq. Ivor R.
Fredman Jeffrey
Mintz Levin Cohn Ferris Glovsky and Popeo PC
Thomas David C.
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