Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2011-04-26
2011-04-26
Carlson, Karen Cochrane (Department: 1657)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
Reexamination Certificate
active
07932080
ABSTRACT:
An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The “double-brilliance” biosensor sandwiches β-arrestin (β-arr) betweenRenillaluciferase (Luc) and the yellow fluorescent protein (YFP). β-arr associates with G-protein coupled receptors GPCR following receptor activation, bringing Luc and YPF into close proximity that favours energy transfer. In addition to providing new insights into the agonist-induced conformational rearrangements of β-arr in living cells, the double-brilliance β-arr offers a universal biosensor for GPCR activation, allowing the study of native receptors in large-scale screening analysis. The activity of other signalling molecules known to interact with β arrestin could also be monitored by double brilliance β arr.
REFERENCES:
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Bouvier Michel
Charest Pascale
Carlson Karen Cochrane
Quarles & Brady LLP
Shen Bin
Valorisation-Recherche, Limited Partnership
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