Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-08-09
1985-04-09
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 21, 435810, 436540, 436548, 436808, G01N 3354
Patent
active
045102392
ABSTRACT:
A method, kit and a solution for assaying enzyme, such as prostate acid phosphatase (PAP), in a sample. The sample is contacted with a first antibody (Ab.sub.1) against the enzyme and incubated. The resulting Ab.sub.1 -enzyme complex is contacted with a second antibody (Ab.sub.2) specific for Ab.sub.1. The resulting Ab.sub.2 -Ab.sub.1 -enzyme complex precipitates out of the medium. The precipitated complex is separated and solubilized with a pH controlled solution of gamma globulin. The resulting solution is then assayed for enzyme activity.
REFERENCES:
patent: 4248964 (1981-02-01), McDonald
patent: 4267272 (1981-05-01), Josephson
patent: 4298592 (1981-11-01), Lin
B. K. Choe et al., Clin. Chem., 26(13), 1854-1859, (1980).
Chemical Abstracts, 97: 211552x, (1982).
Miller Steven P.
Quan Stella S. M.
Beckman Instruments Inc.
Frieman R. S.
Marantz Sidney
Meads R. R.
Steinmeyer R. J.
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