Dopamine-.beta.-hydroxylase determination method

Details Dopamine-.beta.-hydroxylase determination method Dopamine-.beta.-hydroxylase determination method

1975-12-04

1977-06-28

Shapiro, Lionel M.

Textiles: fiber preparation

G01N 3114

Patent

active

040324060

ABSTRACT:
A method of determining dopamine-.beta.-hydroxylase (D.beta.H) activity in biological fluids which comprises reacting a D.beta.H-specific substrate with a sample of said biological fluid in the presence of ascorbate, fumarate and oxygen and measuring the uptake of oxygen by the resulting hydroxylation with an oxygen-sensing electrode.

REFERENCES:
patent: 3838011 (1974-09-01), Hagen et al.
patent: 3857771 (1974-12-01), Sternberg
Nagatsu et al., "Photometric Assay of Dopamine-.beta.-Hydroxylase Activity in Human Blood", Clin. Chem., vol. 18, No. 9 (1972) pp. 980-983.
Goldstein et al., "Kinetic Studies of the Enzymatic Dopamine .beta.-Hydroxylation Reaction", Biochemistry, vol. 7, No. 8 (1968) pp. 2724-2730.
Kadish et al., "A New and Rapid Method for the Determination of Glucose by Measurement of Rate of Oxygen Comsumption", Clin. Chem., 14-2 (1968), pp. 116-131.
Sweetman et al., "The Oxidation of Biologically Important Monamines by Monamine Oxidase", Exp. in Physiol. & Biochem. (1972) 5, pp. 303-328.

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