DNAs encoding analog GM-CSF molecules displaying resistance to p

Details DNAs encoding analog GM-CSF molecules displaying resistance to p DNAs encoding analog GM-CSF molecules displaying resistance to p

1985-08-06

1995-02-21

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352401, 4352542, 4353201, 536 235, C12N 1527, C12N 1581, C12N 119

Patent

active

053914856

ABSTRACT:
Amplified expression of recombinant DNA products is achieved in hosts expressing proteases that cleave at multi-basic amino acid residues. To this end, cDNAs encoding granulocyte-macrophage colony stimulating factor (GM-CSF) are mutated such that one or both of the arginine residues at positions 23 and 24 of the protein product are replaced by non-basic amino acid residues. The GM-CSF analogs thus obtained maintain the activity of the wild-type protein.

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