Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-12-05
1991-05-14
Mays, Thomas
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 91, 4351723, 435226, 43525233, 4353201, 935 29, 935 42, 935 72, 935 73, C12P 2100, C12P 1934, C12P 1500, C12P 120
Patent
active
050155733
ABSTRACT:
A new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.
REFERENCES:
patent: 4806471 (1989-02-01), Molin et al.
Caulcott Celia A.
Humphreys Gwynfor O.
Robinson Martin K.
Wright Edwina M.
Yarranton Geoffrey T.
Celltech Limited
Mays Thomas
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