DNA vectors and their use in recombinant DNA technology

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 91, 4351723, 435226, 43525233, 4353201, 935 29, 935 42, 935 72, 935 73, C12P 2100, C12P 1934, C12P 1500, C12P 120

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050155733

ABSTRACT:
A new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.

REFERENCES:
patent: 4806471 (1989-02-01), Molin et al.

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