DNA vaccine for Koi herpes virus (KHV) disease

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...

Reexamination Certificate

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C435S005000

Reexamination Certificate

active

08052977

ABSTRACT:
The present invention provides a DNA vaccine for carps for inducing protective immunity against Koi herpesvirus (KHV). The DNA vaccine comprises a DNA comprising a nucleotide sequence encoding an immunogenic polypeptide against Koi herpesvirus (KHV) of carps, or an expression vector comprising the DNA as an active ingredient.

REFERENCES:
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patent: 2003-155254 (2003-05-01), None
patent: 2005-112726 (2005-04-01), None
Gray et al, Journal of Fish Diseases, 2002, vol. 25, pp. 171-178.
Boudinot P. et al., “Combined DNA immunization with the glycoprotein gene of viral hemorrhagic septicemia Virus and infectious hematopoietic necrosis Virus induces double-specific protective immunity and nonspecific response in rainbow trout,” Virology, 1998, vol. 249, p. 297-306.
Database DDBJ/EMBL/GenBank online, Accession No. Q75N32, <<http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?75553604:CAGE—DDBJ:1316278>> Jul. 5, 2004 uploaded, Kurita J. et al., Definition: Hypothetical protein.
Database DDBJ/EMBL/GenBank online, Accession No. Q75N31, <<http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?75553603:CAGE—DDBJ:1316277>> Jul. 5, 2004 uploaded, Kurita J. et al., Definition: Membrane protein.
Database DDBJ/EMBL/GenBank online, Accession No. Q75N28, <<http://www.ncbi.nlm.nih.gov/entrez/viewerfcgi?75553600:CAGE—DDBJ:1316 274>> Feb. 1, 2005 uploaded, Kurita J. et al., Definition: Hypothetical protein.
Database DDBJ/EMBL/GenBank online, Accession No. Q75N51, <<http://www.ncbi.nlm.nih.gov/entrez/viewerfcgi?75553606:CAGE—DDBJ:1316 280>> Jul. 5, 2004 uploaded, Kurita J. et al., Definition: Membrane protein.
Waltzek et al., “Koi herpesvirus represents a third cyprinid herpesvirus (Cy1-1V-3) in the familyHerpesviridae,” Journal of General Virology, 86, pp. 1659-1667, 2005.
Ronen et al., “Efficient vaccine against the virus causing a lethal disease in culturedCyprinus Carpio,” Vaccine 21, pp. 4677-4684, 2003.
Kurita et al., “Cyprinid herpesvirus 3 ORF1, ORF2, ORF3, ORF4, ORF5 genes for hypothetical protein, membrane protein, membrane protein, major envelope protein, hypothetical protein, complete cds, strain: KHVO301,” database EMBL (Online), XP002602385 Database accession No.EM—VI:AB178537, May 10, 2004.
Kurita et al., “Cyprinid herpesvirus 3 genes for membrane protein, major envelope protein, hypothetical protein, complete cds,” database EMBL (Online), XP002602386 Database accession No. EM—VI:AB178324, May 1, 2004.
European Search Report issued in European Application No. 07707716.2, dated Oct. 15, 2010.

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