Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1991-01-31
1994-11-15
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436 94, 536 2431, 935 77, 935 78, C12Q 168, C07H 1512, G01N 3348, G01N 33566
Patent
active
053647599
ABSTRACT:
The present invention relates to a DNA profiling assay for detecting polymorphisms in a short tandem repeat. The method includes the steps of extracting DNA from a sample to be tested, amplifying the extracted DNA and identifying the amplified extension products for each different sequence. Each different sequence is differentially labeled. In the method, internal and external standards can also be used. The method is applicable to a wide variety of forensic and medical samples, including blood, semen, vaginal swaps, tissue, hair, saliva, urine and mixtures of body fluids. A short tandem repeat sequence which can be characterized by the formula (A.sub.w G.sub.x T.sub.y C.sub.z).sub.n, wherein A, G, T and C represent the nucleotides, w, x, y and z represent the number of nucleotide and range from 0 to 7 and the sum of w+x+y+z ranges from 3 to 7 and n represents the repeat number and ranges from 5 to 50. The labels can be from a variety of groups, including fluorescers, radioisotopes, chemiluminescers, stains, enzymes and antibodies. Also described is a kit. Further, a method of detecting the polymorphic short tandem repeats comprising the steps of either searching for the repeats in a data base or comparing oligonucleotides and searching for the repeats in a genetic library.
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Caskey Charles T.
Edwards Albert O.
Baylor College of Medicine
Jacobson Dian C.
Wax Robert A.
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