DNA transposon TN5SUPF in plasmid pBRG1310

Details DNA transposon TN5SUPF in plasmid pBRG1310 DNA transposon TN5SUPF in plasmid pBRG1310

1990-01-22

1994-05-31

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

4351723, 43525233, 536 242, C12N 1563, C12N 1570

Patent

active

053169468

ABSTRACT:
A small, novel transposon useful for mutagenesis and sequencing DNAs cloned in phage .lambda. is disclosed which comprises a transposon having at each terminus a segment of 19 nucleotides selected from the group consisting of the O-end and I-end sequences of Tn5, at least one restriction enzyme site positioned less that 20 nucleotides distant from each said terminal segment, and a supF amber-suppressor tRNA gene insert.

REFERENCES:
patent: 4716105 (1987-12-01), Mizuuchi et al.
Seed et al. (1983), Nucleic Acid Research, vol. 11, pp. 2427-2445.
Vermersch et al. (1987), Gene, vol. 54, pp. 229-238.
Ubben et al. (1986), Gene, vol. 41, pp. 145-152.
Phadnis et al. (1987), Proc. Natl. Acad. Sci., vol. 84, pp. 9118-9122.
Levinson et al. (1984), J. Mol. App. Genet.; vol. 2, Abstract only.
Berg et al., Genetics 105, 813-828 (1983).
Berg and Berg, Bio/Technology 1, 417-435 (1983).
Berg and Berg, in Neidhardt et al., E. coli and S. typhimurium: Cellular and Mol. Biol., 1071-1109 (1987).
Johnson and Resnikoff, Nature 304, 280-282 (1983).
Sasakawa et al., Proc. Natl. Acad. Sci. U.S.A. 80, 7293-7297 (1983).
Auserwald et al., Cold Spring Harbor Symp. Quant. Biol. 45, 107-113 (1983).
Krebs and Reznikoff, J. Mol. Biol. 192, 781-791 (1986).
Melton et al., Nucl. Acids. Res. 12, 7035-7056 (1984).
Studier and Moffatt, J. Mol. Biol. 189, 113-130 (1986).
Egelhoff et al., DNA 4, 241-248 (1985).
Phadnis, Huang & Berg, Proc. Natl. Acad. Sci. U.S.A. 86, 5908-5912 (1989).
Berg, in Mobile DNA, eds. Berg & Howe (ASM), Ch. 6, pp. 185-210 (1989).

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