DNA transposon TN5SEQ1

Details DNA transposon TN5SEQ1 DNA transposon TN5SEQ1

1987-10-05

1992-08-11

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Vector, per se

4351721, 4351723, 935 29, C12N 1563, C12N 1509

Patent

active

051378299

ABSTRACT:
A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.

REFERENCES:
patent: 4656134 (1987-04-01), Ringold
patent: 4683202 (1987-07-01), Mullis
patent: 4716105 (1987-12-01), Mizuuchi et al.
Nag et al. Gene 64:135-145 (1988).
Berg et al., Genetics 105, 813-828 (1983).
Berg and Berg, Bio/Technology 1, 417-435 (1983).
Berg and Berg, in Neidhardt et al. E. coli and S. typhimurium: Cellular and Mol. Biol. 1071-1109 (1987).
Johnson and Reznikoff, Nature 304, 280-282 (1983).
Sasakawa et al., Proc. Natl. Acad. Sci. USA 80, 7293-7297 (1983).
Auserwald et al., Cold Spring Harbor Symp. Quant. Biol. 45, 107-113 (1983).
Krebs and Reznikoff, J. Mol. Biol. 192, 781-791 (1986).
Melton et al., Nucl. Acids. Res. 12, 7035-7056 (1984).
Studier and Moffatt, J. Mol. Biol. 189, 113-130 (1986).
Egelhoff et al., DNA 4, 241-248 (1985).

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