DNA transfer vector for gram-negative bacteria

Details DNA transfer vector for gram-negative bacteria DNA transfer vector for gram-negative bacteria

1983-07-01

1986-12-02

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435317, 935 29, 935 30, 935 55, 935 67, 935 72, C12N 1500, C12N 120, C12N 100

Patent

active

046265040

ABSTRACT:
Modified E. coli vectors are disclosed that are mobilizable to a wide range of gram-negative bacteria, but are not self-transmissible. The modified E. coli plasmids replicate only in bacterial strains of the E. coli group. Mobilizer strains of E. coli are also provided, as well as methods for employing the modified E. coli vectors for transposon mutagenesis, site-specific gene transfer, and the construction of DNA libraries.

REFERENCES:
Simon, R. et al., Bio/Technology, Nov., 1983, "A Broad Host Range Mobilization System for in vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria".
Simon, R. et al in Molecular Genetics of the Bacteria-Plant Interaction, (A. Puhler, ed.), pp. 98-106, 1983, Springer-Verlag, publisher.
Simon et al, "Analysis of Plasmid Borne Genes in Rhizobium meliloti by Tn5 Mutagenesis", Winnipeg Symposium-Proc. 8th N. Amer. Rhizobium Conf. (meeting held in 1981).
Bagdassarian et al, Gene, vol. 16, pp. 237-242 (1981).
Bagdassarian et al., Microbial Drug Resistance, Proc. 3rd Int'l Simp., Tokyo, S. Mitshuhashi (ed) pp. 183-197 (1982).
Chang, et al, J. Bact., vol. 134, pp. 1141-1156 (1978).
8th North American Rhizobium Conference Abstract Book, pp. 1-3, 7-15, 20, 22, 25, 28-29, 42-43, 100-103, 105, 128, 130, 132 and 136 (Aug. 3-7, 1981).
Proceedings, 8th North American Rhizobium Conference, pp. 90-114 (K. W. Clark & J. H. G. Stephens, eds., 1983).
Datta, N., et al. (1971) J. Bact., 108:1244-1249.
Datta, N. and R. W. Hedges (1972) J. Gen. Microbiol., 70:453-460.
Olsen, R. H. and P. Shipley (1973) J. Bact., 113:772-780.
Beringer, J. G. (1974) J. Gen. Microbiol., 84:188-198.
Beringer, J. E., et al. (1978) Nature, 276:633-634.
Priefer, U. B., et al. (1980) In Antibiotic Resistance, Transposition and Other Mechanisms (S. Mitshuhashi, L. Rosival and V. Krcmery, eds.) Springer-Verlag, Berlin, pp. 91-96.
Simon, R. (1980) In Antibiotic Resistance, Transposition and Other Mechanisms (S. Mitshuhashi, L. Rosival and V. Krcmery, eds.) Springer-Verlag, Berlin, 35-42.

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