Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2005-10-04
2005-10-04
Housel, James (Department: 1648)
Chemistry: molecular biology and microbiology
Vector, per se
C435S069100, C435S440000, C435S456000, C435S465000, C435S471000, C435S475000, C435S173300, C435S235100, C536S023100, C536S023720, C536S024100
Reexamination Certificate
active
06951754
ABSTRACT:
The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuramimidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus. Attenuated or inactivated RNA viruses produced by the present invention may be administered to a patient in need of vaccination by any of several routes including intranasally or intramuscularly.
REFERENCES:
patent: 3992522 (1976-11-01), Chanock et al.
patent: WO 91/03552 (1991-03-01), None
patent: WO 00/60050 (2000-10-01), None
Pleschka J Virology 1996 vol. 70, pp. 4188-4192.
Pekosz Proc. Nat. Acad. Sci, USA Aug. 1999, vol. 96, pp. 8804-8806.
Neumann 1999 (Aug.) vol. 96, pp. 9345-9350.
Hoffmann 1997 Generation of an RNA-Polymerase Vector System for the Selective Mutagenesis of Influenza A (in translation).
Erich Hoffmann et al., “‘Ambisense’ Approach for the Generation of Influenza A Virus: vRNA and mRNA Synthesis from One Template,” Virology, 1999, vol. 267, pp. 310-317.
Erich Hoffmann et al., “Characterization of the Influenza A Virus Gene Pool in Avian Species in Southern China: Was H6N1 a Derivative or a Precursor of H5N1?” J. Virology, 2000, vol. 74, No. 14, pp. 6309-6315.
Flick, et al., “Promoter elements in the influenza vRNA terminal structure,” RNA, 1996; 2(10):1046-1057.
Luytjes, et al., “Amplification, Expression, and Packaging of a Foreign Gene by Influenza Virus,” Cell, 1989; 59(6):1107-1113.
Neumann, et al., “Genetic Engineering of Influenza and Other Negative-Strand RNA Viruses Containing Segmented Genomes,” Advances in Virus Research, 1999; 53: 265-300.
Neumann, et al., “RNA Polymerase I-Mediated Expression of Influenza Viral RNA Molecules,” Virology, 1994; 202:477-479.
Schnell, et al., “Infectious rabies viruses from cloned cDNA,” EMBO Journal, 1994;13(18):4195-4203.
Scholtissek, et al., “The Nucleoprotein as a Possible Major Factor in Determining Host Specificity of Influenza H3N2 Viruses,” Virology, 1985; 147:287-294.
Zobel, et al., “RNA polymerase I catalysed transcription of insert viral cDNA,” Nucleic Acids Research, 1993; 21(16):3607-3614.
Bergmann, et al., “The relative amount of an influenza A virus segment present in the viral particle is not affected by a reduction in replication of that segment,”, Journal of General Virology, 1995, 76:3211-3215.
Ghendon, “Cold-Adapted, Live Influenza Vaccines Developed in Russia,” Textbook of Influenza,Chapter 29, pp. 391-399.
Nicholson, et al., “Live Cold-Adapted, Reassortant Influenza Vaccines(USA),”Textbook of Influenza,Chapter 28, pp. 373-390.
Hoffmann, Erich, Aufbau eines RNA-Polymerase I-Vektorsystems zur gezielten Mutagenese von Influenza A Viren, Giessen 1997 (Doctoral Dissertation).
Hoffman, et al., “A DNAtransfection system for generation of influenza A virus from eight plasmids”, Proc. Nat. Acad. Sci. U.S.A. 2000, vol. 97, pp. 6108-6113.
Hoffman and Webster, “Unidirectional RNA polymerase 1-polymerase II transcription system for the generation of influenza A virus from eight plasmids,” J. Gen. Virol. 2000, vol. 81, pp. 2843-2847.
Palese, Peter, et al., “Negative-strand RNA Viruses: Genetic Engineering and Applications”, Colloquium Paper, Proc. Natl. Acad. Sci., U.S.A., Oct. 1996, vol. 93, pp. 11354-11358.
Neumann, Gabriele, et al., “Generation of Influenza A Viruses Entirely From Cloned cDNAs”, Microbiology, Proc. Natl. Acad. Sci., U.S.A., Aug. 1999, vol. 96, pp. 9345-9350.
Fodor, Ervin, et al.,“Rescue of Influenza A Virus from Recombinant DNA”, Journal of Virology, American Society for Microbiology, Nov. 1999, vol. 73, No. 11, pp. 9679-9682.
Guan, Yi, et al., “Molecular Characterization of H9N2 Influenza Viruses: Were They The Donors of the “Internal” Genes of H4N1 Viruses in Hong Kong?”, Microbiology, Proc. Natl. Acad. Sci., U.S.A., Aug. 1999, vol. 96, pp. 9363-9367.
Perez, Daniel R. et al., “The Matrix 1 Protein of Influenza A Virus Inhibits The Transciptase Activity of a Model Influenza Reporter Genome in Vivo”, Article No. VY989318, Virology, 1998, vol. 249, pp. 52-61.
Xu, Xiyan, et al.,“Genetic Characterization of the Pathogenic Influenza A/Goose/Guangdong/1/96 (H5N1) Virus: Similarly of its Hemagglutinin Gene to Those of H5N1 Viruses form the 1997 Outbreaks in Hong Kong”, Article ID viro. 1999.9820, Virology, 1999, vol. 261, pp. 15-19.
Durbin, Anna P., et al., “Recovery of Infectious Human Parainfluenza Virus Type 3 from cDNA”, Article No. VY978697, Virology, 1997, vol. 235, pp. 323-332.
Zhou, Yan, et al., “Membrane-Anchored Incorporation of a Foreign Protein in Recombinant Influenza Virions”, Article No. VY989169, Virology, 1998, vol. 246, pp. 83-94.
Govorkova, E.A., et al., “African Green Monkey Kidney(Vero)Cells Provide an Alternative Host Cell System for Influenza A and B Viruses”, Journal of Virology, American Society for Microbiology, Aug. 1996, vol. 70, No. 8, pp. 5519-5524.
Pleschka, Stephan, et al., “A Plasmid-Based Reverse Genetics System for Influenza A Virus”, Journal of Virology, American Society for Microbiology, Jun. 1996, vol. 70, No. 6, pp. 4188-4192.
Muster, Thomas, et al., “An Influenza A Virus Containing Influenza B Virus 5′ and 3′ Noncoding Regions on the Neuraminidase Gene is Attenuated in Mice”, Microbilogy, Proc. Natl. Acad. Sci., U.S.A., Jun. 1991, vol. 88, pp. 5177-5181.
Enami, Masayoshi, et al., “Introduction of Site-specific Mutations into the Genome of Influenza Virus”, Microbiology, Proc. Natl. Acad. Sci., U.S.A., May 1990, vol. 87, pp. 3802-3805.
Darby & Darby
Hill Myron G.
Housel James
St. Jude Children's Research Hospital
LandOfFree
DNA transfection system for the generation of infectious... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA transfection system for the generation of infectious..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA transfection system for the generation of infectious... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3446805