DNA superfragment cloning

Details DNA superfragment cloning DNA superfragment cloning

1991-04-05

1993-08-31

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 91, 536 231, 536 235, C12N 1510, C12N 1511, C12N 1512

Patent

active

052408400

ABSTRACT:
Superfragments of chromosomal DNA having 1 million to 100 million base pairs may be formed by irradiating microcells containing a chromosome with a high dose of .gamma.-irradiation. Such superfragments are useful for the rapid cloning of genes.

REFERENCES:
Goss et al. (Jun. 26, 1975), Nature, vol. 255, pp. 680-684.
Leach et al. (Aug. 1989), Genomics, vol. 5, pp. 167-176.
Reeve et al. (Apr. 1989), Mol. Cell. Biol., vol. 9(4), pp. 1799-1803.
Somnatic Cell and Molecular Genetics, vol. 16, No. 5, pp. 425-435, Sep., 1990, T. S. Siden, et al., "Monochromosomal Mouse Microcell Hybrids Containing Inserted Selectable Neo Genes".

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