DNA strand coding for .alpha.-acetolactate decarboxylase and yea

Details DNA strand coding for .alpha.-acetolactate decarboxylase and yea DNA strand coding for .alpha.-acetolactate decarboxylase and yea

1986-12-11

1990-01-23

Teskin, Robin

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435232, 435320, 435940, 536 27, 426 11, 426 20, C12N 1500, C12N 700, C07H 1312

Patent

active

048958026

ABSTRACT:
DNA strand having an ability in biotechnological production of .alpha.-acetolactate decarboxylase is disclosed. The DNA strand is characterized in that it has a nucleotide sequence coding for a polypeptide whose amino acid sequence is substantially from A to B of FIG. 1 and which has .alpha.-acetolactate decarboxcylase activity. Also disclosed is a yeast which belongs to Saccharomyces cerevisiae and which has been transformed by the DNA strand. The yeast is characterized by the face that its .alpha.-acetolactate producing ability is reduced, and will thus produce an alcoholic liquor such as beer which contains no or little diacetyls which have come from their precursor, namely .alpha.-acetolactate.

REFERENCES:
patent: 4617273 (1986-10-01), Olsen
Carlsberg Res. Commun., vol. 48, 1983, pp. 239-247; S. E. Godtfredsen "On the Occurrence . . . Microorganism" *Whole Article*.
EBC Congress 1983, vol. 89, Lecture No. 17, pp. 161-168; S. E. Godtfresen et al.: "Use of . . . of Beer" * Whole Article*.
Journal of Biotechnology, vol. 5, 1987, pp. 87-91, Elsevier Science Pub. B.V. (Biomedical Division): Sone et al.: "Molecular . . . Aerogenes".
Acetolactate Decarboxylase from Aerobacter Aerogenes Eur. J. Biochem 14:133 (1970) Loken & Stormer.
2, 3 Butanediol Biosynthetic System . . . Stormer & Wood, Methods in Enz. 41:518 (1975).
Use of Acetolactate . . . Proc. Eur. Brewery Convention 1983, p. 161, Godtfredson.

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