DNA sizing control standards for electrophoretic analyses

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435967, 436 8, 536 231, 935 78, C12Q 168

Patent

active

053025105

ABSTRACT:
The present invention is directed to a method for assessing the reliability of DNA sizing measurements performed on DNA samples. DNA sizing control standards are disclosed wherein each standard contains a unique set of DNA restriction fragments composed of coding fragments and measuring fragments of known sizes. Methods for constructing the fragments which can be used in making the DNA sizing control standards are disclosed. Kits comprising the DNA sizing control standards of the present invention are also disclosed.

REFERENCES:
patent: 4771384 (1988-09-01), Daniels et al.
patent: 5030566 (1991-07-01), Son et al.
Carman et al., Detection of Enzymatically Amplified Human Immunodeficiency Virus DNA by Oligonucleotide Solution Hybridization and by Incorporation of Radio- . . . , J. Clin. Microbiol. 27:2570-2573 (1989).
Gralla, J. D., Rapid "footprinting" on supercoiled DNA, Proc. Natl. Acad. Sci. USA 82:3078-3081 (1985).
Molecular and Cell Biology 1990 Price List, p. 23, Pharmacia Inc.
Product insert for DNA Analysis Marker System, marketed by GIBCO BRL, available in 1990.
Bernards et al., Pulsed field gradient electrophoresis of DNA digested in agarose allows the sizing of the large duplication unit of a surface . . . , Chem. Abstr. 105:187 Abstract No. 92470r (1986).
Budowle et al., Modifications to improve the effectiveness of restriction fragment length polymorphism typing, Applied and Theoretical Electrophoresis 1:181-187 (1990).
Huang et al., Restriction endonuclease analysis of granulosis virus DNA of Agrotis exclamationis Linnaeus, Chem. Abstr. 107:175 Abstr. No. 34093a (1987).
Jones et al., Separation of yeast chromosomes in the megabase range suitable as size markers for pulsed-field gel electrophoresis, Chem. Abstr. 112:172 Abtr. No. 230760h (1990).
Mathew et al., High-Resolution Separation and Accurate Size Determination in Pulsed-Field Gel Electrophoresis of DNA. 1. DNA Size Standards and the Effect . . . , Biochemistry 27:9204-9210 (1988).
Promega 1990-1991 Catalog, Chromosomal DNA Analysis 278-279.
Rickwood et al., "Practical Approaches in Biochemistry Series" IRL Press Oxford, pp. 227-232 (1990).
Waterbury et al., Generation of lambda phage concatemers for use as pulsed field electrophoresis size markers, Nucleic Acids Research 15(9):3930 (1987).
English Abstract of European Patent Publication 0 357 028 published Mar. 7, 1990.
1991 Life Technologies Inc. Catalog, p. 325.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

DNA sizing control standards for electrophoretic analyses does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA sizing control standards for electrophoretic analyses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sizing control standards for electrophoretic analyses will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2098125

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.