DNA sequencing with pyrophosphatase

Details DNA sequencing with pyrophosphatase DNA sequencing with pyrophosphatase

1994-07-14

1996-03-12

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 915, 435194, 435195, 435 21, 436 94, 935 77, 935 78, C12Q 168, C12P 1934, G01N 3350, C12N 914

Patent

active

054985234

ABSTRACT:
A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

REFERENCES:
patent: 4572894 (1986-02-01), Imahori et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4795699 (1989-01-01), Tabor
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5001050 (1990-03-01), Blanco
Freifelder, D. Ie; Molecular Biology, (1987) Jones & Bartlett Publishers, Inc., Boston, Mass. pp. 234-235.
Saiki et al. "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science, vol. 239, pp. 487-490, 1988.

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