DNA sequencing method and reagents kit

Details DNA sequencing method and reagents kit DNA sequencing method and reagents kit

1997-10-09

1999-10-19

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1934

Patent

active

059687436

ABSTRACT:
The present invention provides a DNA sequencing method comprising: (1) a step of fragmentation of a sample DNA and amplifying each fragment to obtain a first DNA fragment; (2) a step of obtaining from the first DNA fragment a second DNA fragment substantially complementary to the sample DNA at least at the 3' terminus thereof; and (3) a step of performing an extension reaction of complementary strand, using the sample DNA as a template to produce a third DNA fragment containing a base sequence complementary to the second DNA fragment and having a size longer than that of the second DNA fragment, and using the third DNA fragment as a template for sequencing of the sample DNA. DNA sequencing can be proceeded efficiently with extremely low redundancy.

REFERENCES:
T. Maniatis et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989, Chapter 13, pp. 21-33.
T. Maniatis et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989, Chapter 13, pp. 14-20.
T. Maniatis et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989, Chapter 13, pp. 34-41.
Gene, 176, 1996, "Fragment Walking for long DNA sequencing by using a library as small as 16 primers", K. Okano et al, pp. 231-235.
T. Maniatis et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989, Chapter 5, pp. 62-63.
T. Maniatis et al, Molecular Cloning, Cold Spring Harbor Laboratory Press, 1989, Chapter 5 pp. 56-57.
Kazunori et al, Gene 176:231-235, 1996.

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