DNA sequencing, mapping, and diagnostic processes using hybridiz

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435810, 422 681, 422 69, 422 78, 422 80, 422 8205, 422 8209, 436501, 536 253, 935 77, 935 78, 935 86, 935 87, C12Q 168

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057802327

ABSTRACT:
A DNA sequencing, mapping, and diagnostic process which includes the steps of labeling nucleotide segments or peptide nucleic acids (PNAs) with one or more atoms of specific stable or long-lived radioactive isotopes of a selected element that do not normally occur in DNAs, ODNs or PNAs such that the nucleotide segment or PNA has specific stable or long-lived radioactive isotope of a specified selected element at a terminal or an interior position; hybridizing the labeled nucleotide segment or PNA to complementary nucleic acid segments or PNAs which are fixed on a hybridization surface; and, using mass spectrometric techniques, including RIS, to analyze the presence and position of the labeled hybridized nucleotide segments or PNAs which are bound to the fixed nucleotide segments or PNAs.

REFERENCES:
patent: 5064754 (1991-11-01), Mills
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5547835 (1996-08-01), Koster

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