DNA sequencing, mapping, and diagnostic processes using hybridiz

Details DNA sequencing, mapping, and diagnostic processes using hybridiz DNA sequencing, mapping, and diagnostic processes using hybridiz

1996-08-02

1998-10-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 4352872, 4352887, 436173, 530300, 536 243, 250282, 250423P, C12Q 168, G01N 3348, B01D 5944

Patent

active

058210601

ABSTRACT:
A process for deoxyribonucleic acid (DNA) sequencing, mapping, and diagnostics which utilizes the differences between the chemical composition of DNA and that of peptide nucleic acids (PNAs) to provide DNA sequencing, mapping, or diagnostics using natural DNA fragments, rather than using radioisotopes, stable isotopes or fluorescent substances to label the DNAs. The process includes the steps of hybridizing PNA segments to complementary DNA segments which are fixed to a hybridization surface, or hybridizing DNA segments to complementary PNA segments which are fixed to a hybridization surface, and using mass spectrometric or non-mass spectrometric techniques to analyze the extent of hybridization at each potential hybridization site.

REFERENCES:
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5547835 (1996-08-01), Koster et al.
patent: 5580733 (1996-12-01), Levis et al.
Peter E. Nielsen, Michael Egholm, Rolf H. Berg, and Ole Buchardt, "Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide", Science 254, pp. 1496-1500, (Dec. 1991).
Michael Egholm, Ole Buchardt, Leif Christensen, Carsten Behrens, Susan M. Freier, David A. Driver, Rolf H. Berg, Seog K. Kim, Bengt Norden & Peter E. Nielsen, "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-Bonding Rules", Nature 365, pp. 566-568, (Oct. 1993).
Wittung et al., Nucleic Acids Research 22(24):5371-5377 1994.
Wittung et al., Nature 368:561-563 Apr. 1994.

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