DNA sequencing gel reading system and method

Radiant energy – Automatic/serial detection of similar sources

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250328, G01T 100

Patent

active

048230073

ABSTRACT:
In place of the conventional photographic DNA sequencing gel method, the present disclosure proposes directly scanning the radio labeled DNA fragments of successive information tracks on the gel to produce an on-line contemporaneous display. In this way, the scanning and/or display may be modified during the progress of the scanning operation, for example to emphasize the development of selected portions of the gel which exhibit relatively faint radioactivity. The scanning operation preferably takes place by a constant speed rotation of the gel and an arcuate scanning of a radioactive detector head with the schedule of angular velocity of the detector varying to maintain a relatively constant relative scanning velocity between the detector head and successive points on the surface of the gel. The direct electronic storage of the gel track configuration results in a facilitation of the reading of the gel in comparison with the laborious procedure required for visually reading the DNA sequence from a photograph of the gel.

REFERENCES:
patent: 4110615 (1978-08-01), McCann

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