DNA sequencing by mass spectrometry via exonuclease degradation

Details DNA sequencing by mass spectrometry via exonuclease degradation DNA sequencing by mass spectrometry via exonuclease degradation

1995-02-10

1997-04-22

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

250282, 250288, 422 681, C12Q 168, B01D 5944, G01N 3072

Patent

active

056228244

ABSTRACT:
Methods for determining the sequence of nucleic acids by cleaving the nueleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nueleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simultaneously determining more than one nucleic acid sequence.

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