DNA sequencing apparatus

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Automatic analytical monitor and control of industrial process

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422 67, 422 8205, 435291, 436 89, C12P 1934, G01N 3300

Patent

active

052215180

ABSTRACT:
The DNA sequencing apparatus contains the following components. A first reaction vessel contains a reaction chamber into and out of which can be transferred reagents, reactants and reaction products. A device for separating individual oligonucleotides and polynucleotides on the basis of length, such as an electrophoretic unit, receives reaction products from the first reaction vessel. A second reaction vessel is designed for oxidizing pentose sugars. It comprises a reaction chamber having a device for transferring reactants and reagents into the reaction chamber and gaseous by-products of a reaction out of the reaction chamber. Separated oligonucleotides and polynucleotides can be selectably transferred from the separating device alternatively into the first or the second reaction vessel. The device also includes a second transfer device for transferring the gaseous by-products out of the second reaction vessel and a collection chamber for collecting the gaseous by-products. The collection chamber is in communication with the second transfer device. Finally, the apparatus contains an analyzer for analyzing the relative abundance of the components of the gaseous by-product by mass such as a mass spectrometer, the analyzer being in communication with a transfer device for transferring gaseous by-products from the collection chamber to the analyzer.

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patent: 4863849 (1989-09-01), Melamede
patent: 4942124 (1990-07-01), Church
patent: 5006465 (1991-04-01), Anderson et al.
patent: 5104621 (1992-04-01), Pfost et al.
patent: 5106585 (1992-04-01), Minami et al.

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