Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1995-12-11
2000-11-07
Woodward, Michael P.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204456, 204461, 204466, 204606, 204612, 204601, 204616, 935 19, C25B 700
Patent
active
061431516
ABSTRACT:
To sequence long strands of DNA, clone strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotin. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the non-biotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method of by ethidium bromide.
REFERENCES:
patent: 3423207 (1969-01-01), Heseltine et al.
patent: 3873432 (1975-03-01), Israel et al.
patent: 3969218 (1976-07-01), Scott
patent: 4111785 (1978-09-01), Roskam
patent: 4138551 (1979-02-01), Steiger et al.
patent: 4166105 (1979-08-01), Hirschfeld
patent: 4256834 (1981-03-01), Zuk et al.
patent: 4284491 (1981-08-01), Vesterberg
patent: 4337063 (1982-06-01), Mihara et al.
patent: 4351709 (1982-09-01), Goetz
patent: 4375401 (1983-03-01), Catsimpoolas
patent: 4404289 (1983-09-01), Masuda et al.
patent: 4405711 (1983-09-01), Masuda et al.
patent: 4414325 (1983-11-01), Masuda et al.
patent: 4707235 (1987-11-01), Englert et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4729947 (1988-03-01), Middendorf et al.
patent: 4804748 (1989-02-01), Seela
patent: 4810348 (1989-03-01), Sarrine et al.
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4830786 (1989-05-01), Pease et al.
patent: 4833332 (1989-05-01), Robertson, Jr. et al.
patent: 4855225 (1989-08-01), Fung et al.
patent: 4870004 (1989-09-01), Conroy et al.
patent: 4910300 (1990-03-01), Urdea et al.
patent: 4948882 (1990-08-01), Ruth
patent: 4981977 (1991-01-01), Southwick et al.
patent: 5039818 (1991-08-01), Pease et al.
patent: 5047519 (1991-09-01), Hobbs, Jr. et al.
patent: 5091519 (1992-02-01), Cruickshank
patent: 5106990 (1992-04-01), Ohno et al.
patent: 5151507 (1992-09-01), Hobbs, Jr. et al.
patent: 5171534 (1992-12-01), Smith et al.
patent: 5187085 (1993-02-01), Lee
patent: 5188934 (1993-02-01), Menchen et al.
patent: 5241060 (1993-08-01), Engelhardt et al.
patent: 5242796 (1993-09-01), Prober et al.
patent: 5268486 (1993-12-01), Waggoner et al.
patent: 5276143 (1994-01-01), Sabesan et al.
patent: 5306618 (1994-04-01), Prober et al.
patent: 5310922 (1994-05-01), Pease et al.
patent: 5326692 (1994-07-01), Brinkley et al.
patent: 5328824 (1994-07-01), Ward et al.
patent: 5329019 (1994-07-01), Pease et al.
patent: 5332666 (1994-07-01), Prober et al.
patent: 5346603 (1994-09-01), Middendorf et al.
patent: 5366860 (1994-11-01), Bergot et al.
"A universal nucleoside for use at ambiguous sites in DNA primers"; R. Nichols, P.C. Andrews, P. Zhang & D. E,. Bergstrom; Nature; vol. 369, Jun. 9, 1994, pp. 492-493.
No year given "5-Nitroindole as an universal base analogue"; D. Loakes & D.M. Brown; Nucleic Acids Research; vol. 22, No. 20; pp. 4039-4043.
Synthesis, Structure, and Deoxyribonucleic Acid Sequencing with a Universal Nucleoside: 1-(2'-Deoxy-BD-ribofuranosyl)-3-nitropyrrole; Donald E. Bergstrom, Peiming Zhang, Pascal H. Toma, Philip C. Andrews, and Ruthann Nichols; American Chemical Society; vol. 117, No. 4, 1995; pp. 1201-1209.
"Aromatic Nonpolar Nucleosides as Hydrophobic Isosteres of Pyrimidine and Purine Nucleosides"; Barbara A. Schweitzer and Eric T. Kool; J. Org. Chem., 1994, vol. 59, No. 24; pp. 7238-7242.
"New Virtual Nucleotide VN.TM.) Phosphoramidite and CPG Labeling Reagents"; Clontechniques Oct. 1994, pp. 12-13.
"Universal Nucleoside"; The Glen Report; vol. 7, No. 1; Sep. 1994.
"Spectral Characterization and Evaluation of Modified Near-Infrared Laser Dyes for DNA Sequencing" D. Shealy, R. Lohrmann, J. Ruth, N. Narayanan, S. Sutter, G. Casay, L. Evans, G. Patonay; Applied Spectroscopy vol. 49, No. 12, 1995; pp. 1815-1820.
"Steady-State and Picosecond Laser Fluorescence Studies of Nonradiative Pathways in Tricarbocyanine Dyes: Implications to the Design of Near-IR Fluorochromes with High Fluorescence Efficiencies"; S. Soper and Q. Mattingly; American Chemical Society 1994, pp. 3744-3752.
"Semiconductor Laser Fluorimetry in the Near-Infrared Region" by T. Imasaka, A Yoshitake and N. Ishibashi; American Chemical Society, 1984, pp. 1077-1079.
"Phase Fluorometry Using a Continuously Modulated Laser Diode" by R. Thompson, J. Frisoli and J. Lakowicz; American Chemical Society 1992; pp. 2075-2078.
"Solid-State Diode Laser-Induced Fluorescence Detection in High-Performance Liquid Chromatograpy" by S. Rahavendran and H. Karnes; Pharmaceutical Research vol. 10, No. 3, 1993, pp. 328-334.
"Near-Infrared Fluorogenic Labels: New Approach to an Old Problem" by G. Patonay and M. Antoine; Analytical Chemistry, vol. 63, No. 6, Mar. 15, 1991; pp. 321A-327A.
"Semiconductor laser-induced fluorescence detection in capillary electro-phoresis using a cyanine dye" by F. Chen, A. Tusak, S. Pentoney, K. Konrad, C. Lew, E. Koh and J. Sternberg; Journal of Chromatograpy A, 652 (1993); pp. 355-360.
"Construction of a short-wave near infrared spectrofluorometer with diode laser source and charge-coupled device (CCD) detection" by J. Silzel and R. Obremski; SPIE vol. 1885 Advances in Fluorescence Sensing Technology 1993; pp. 439-447.
"A versatile infrared laser scanner/electrophoresis apparatus" by L. Middendorf, J. Bruce, R. Bruce, R. Eckles, S. Roemer, and G. Sloniker; SPIE vol. 1885 Advances in Fluorescence Sensing Technology (1993) pp. 423-434.
"Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus" by L. Middendorf, J. Bruce, R. Bruce, R. Eckles, D. Grone, S. Roemer, G. Sloniker, D. Steffens, S. Sutter, J. Brumbaugh, G. Patonay; Electrophoresis 1992, 13, pp. 487-494.
"Convenient Methods of Labeling Nucleic Acids for Sequencing with Long Wavelength Dyes" by E. Stirchak, E. Anderson, S. Bourassa, N. Reimer, and C. Brush; Human Genome Project: Commercial Implications, (1994).
"Automated Sequencing using a Carbocyanine Dye" by C. Brush, E. Stirchak, L. Conrad, J. Vornsand, H. Voss, W. Ansorge and R. Duthie; Human Genome: Commercial Implications, (1994).
"Cyanine Dye Labeling Reagents Containing Isothiocyanate Groups" by R. Mujumdar, L. Ernst, S. Mujumdar and A. Waggoner; Cytometry 10:11-19 (1989).
"Cyanine Dye Labeling Reagents: Sulfoindocyanine Succinimidyl Esters" by R. Mujumdar, L. Ernst, S. Mujumdar, C. Lewis and A. Waggoner; Bio-conjugate Chemistry Mar./Apr. 1993, vol. 4, No. 2; pp. 105-111.
No year given Chemistry: Inorganic, Organic, and Biological Chapters 12--Organic Chemistry; 13--Aliphatic Hydrocarbons; 14--Halogen Derivatives, Alcohols, Ethers, Aldehydes, and Ketones; 15--Organic Acids, Organic Salts, Esters, Amides, and Amines; 16--Cyclic Organic Compounds; 17--Carbohydrates; 18--Lipids; 19--Proteins; 20--Nucleoproteins and Nucleic Acids; pp. 58-128.
"Synthesis and duplex stability of oligonucleotides containing cytosine-thymine analogues" by P. Lin and D. Brown; Nucleic Acids Research; vol. 17, No. 24, 1989; pp. 10373-10383.
"Flexible Aglycone Residues in Duplex DNA" by P. Francois, D. Perilleux, Y. Kempener and E. Sonveaux; Tetrahedron Letters vol. 31, No. 44, pp. 6347-6350, 1990.
"Synthesis and duplex stability of oligonucleotides containing adenine-quanine analogues" by D. Brown and P. Lin; Carbohydrate Research; 216, 1991; pp. 129-139.
"Synthesis and biophysical studies of short oligodeoxynucleotides with novel modifications: a possible approach to the problem of mixed base oligodeozynucleotide synthesis" by T. Millican, G. Mock, M. Chauncey, T. Patel, M. Eaton, J. Gunning, S. Cutbush, S. Neidle and J. Mann; Nucleic Acids Research vol. 12, No. 19, 1984; pp. 7345-7453.
"An Alternative to the Mixed Probe Method in DNA Hybridization: Synthetic "lure
Brumbaugh John
Middendorf Lyle Richard
Carney Vincent L.
Delacroix-Muirheid C.
Li-Cor Inc.
Woodward Michael P.
LandOfFree
DNA sequencing does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA sequencing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sequencing will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1637210