DNA sequencing

Details DNA sequencing DNA sequencing

1995-04-13

1997-10-07

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 5, 435 912, 435 915, 435 21, 435194, 435195, 2041828, 204299, 422116, 422 681, 536 243, 536 2432, 536 2433, C12P 1934, C12Q 168, C12Q 170, C07K 314

Patent

active

056747160

ABSTRACT:
A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

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