DNA sequences for the expression of alloproteins

Details DNA sequences for the expression of alloproteins DNA sequences for the expression of alloproteins

2009-06-19

2010-02-23

Wang, Andrew (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C435S199000, C435S325000, C536S023200

Reexamination Certificate

active

07666640

ABSTRACT:
The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating inE. coliwith an enhanced specificity for a non-naturally-occurring tyrosine derivative as compared with the specificity for tyrosine; (B) suppressor tRNA originating inBacillusspecies,Mycoplasmaspecies orStaphylococcusspecies of eubacteria and capable of binding with the tyrosine derivative in the presence of the mutant tyrosyl tRNA synthetase; and, (C) a desired protein gene that has undergone a nonsense mutation at a desired site; wherein, the tyrosine derivative is incorporated at the location of this nonsense mutation.

REFERENCES:
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