Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-01-05
1998-02-17
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352523, 4352551, 4353201, 435325, 435419, 536 236, 800205, A01H 500, C12N 119, C12N 121, C12N 1511, C12N 1563
Patent
active
057190437
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to DNA sequences that contain the coding region of amino acid transporters, whose introduction in a plant genome modifies the transfer of metabolites in transgenic plants, plasmids, bacteria, yeasts and plants containing these DNA sequences, as well as their use.
For many plant species it is known that the delivery of energy-rich compounds to the phloem through the cell wall takes place throughout the cell. Transporter molecules which allow the penetration of amino acids through the plant cell wall are not known.
In bacteria, numerous amino acid transport systems have been characterized. For aromatic amino acids, 5 different transporters have been described which can transport any one of phenylalanine, tyrosine and tryptophan, while the other transporters are specific for individual amino acids (see Sarsero et al., 1991, J Bacteriol 173: 3231-3234). The speed constants of the transport process indicates that the specific transport is less efficient. For several transporter proteins, the corresponding genes have been cloned. This has been achieved using transport-deficient mutants which were selected for their transport ability after transformation with DNA fragments as inserts in expression vectors (see Wallace et al., 1990, J Bacteriol 172: 3214-3220). The mutants were selected depending on their ability to grow in the presence of toxic analogues of amino acids, since the mutants cannot take these up and therefore cannot be impaired.
Corresponding complementation studies have been carried out with the eukaryotic yeast, Saccharomyces cerevisiae. Tanaka & Fink (1985, Gene 38: 205-214) describe a histidine transporter that was identified by complementation of a mutation. Vandenbol et al. (1989, Gene 83: 153-159) describe a proline transporter for Saccharomyces cerevisiae. The yeast possesses two different permeases for proline. One transports with lower efficiency and can be used also for other amino acids, and the other is proline-specific and works with high affinity. The latter was coded from the put4 gene. This carries an open reading frame for a peptide with a molecular weight of 69 kDa. The protein contains 12 membrane-penetrating regions, but does not contain any N-terminal signal sequence for secretion. This is a typical property of integral membrane proteins. The permeases process homology for arginine and for histidine permease from yeast, but not, however, for proline permease from Escherichia coli.
For plant cells, based on studies on tobacco suspension cultures, it has been found that the transport of arginine, asparagine, phenylalanine and histidine are pH and energy dependent. Since a 1,000-fold excess of leucine inhibits the transport of the other amino acids, it can be assumed, therefore, that all amino acids use the same transporter (McDaniel et al., 1982, Plant Physio 69: 246-249). Li and Bush (1991, Plant Physiol 96: 1338-1344) determined, for aliphatic, neutral amino acids, two transport systems in plasma membrane vesicles from Beta vulgaris. On the one hand, alanine, methionine, glutamine and leucine displace each other on the transporter protein. On the other hand, isoleucine, valine and threonine have mutually competitive effects. In combined competition kinetic studies (Li & Bush, 1990, Plant Physiol 94: 268-277) four different transport systems have been distinguished. Besides a transporter for all neutral amino acids, which work with low affinity, there exists a high affinity type which, however, possesses low affinity for isoleucine, threonine, valine and proline. Further transporters exist for acids as well as for basic amino acids.
The transporter molecule or gene for plant transporter proteins is not known.
SUMMARY OF THE INVENTION
There are now described DNA sequences which contain the coding region of a plant amino acid transporter, and whose information contained in the nucleotide sequence allows, by integration in a plant genome, the formation of RNA, by which a new amino acid transport
REFERENCES:
Li, Z.-C., et al., "Delta pH-Dependent Amino Acid Transport Into Plasma Membrane Vesicles Isolated From Sugar Beet Leaves", Plant Physiology, vol. 94, 1990, pp. 268-277.
Li, Z.-C., et al., "Delta pH-Dependent Amino Acid Transport Into Plasma Membrane Vesicles Isolated From Sugar Beet (Beta vulgaris L.) Leaves", Plant Physiology, vol. 96, 1991, pp. 1338-1344.
Tanaka, J., et al., "The Histidine Permease Gene HIP1 of Saccharomyces cerevisiae", Gene, vol. 38, 1985, pp. 205-214.
EMBL Sequence Database, Acc. No. X67124 Rel. 35, 28 Feb. 1993, A. Thaliana PPP mRNA for Amino Acid Permease I.
Frommer, W.B., et al., "Expression Cloning in Yeast of a cDNA Encoding Broad Specificity Amino Acid Permease from Arabidopsis thaliana", Proceedings of the National Academy of Sciences of USA, vol. 90, Jul. 1, 1993, pp. 5944-5948.
Kwart, M., et al., "Differential Expression of Two Related Amino Acid Transporters with Differing Substrate Specificity in Arabidopsis thaliana", The Plant Journal, vol. 4, No. 6, Dec. 1993, pp. 993-1002.
Kwart et al. (1993) The Plant Journal, vol. 4, pp. 993-1002.
Sentenac et al. (1 May 1992) Science vol. 256, pp. 663-665.
Ohnishi et al. (1988) Jpn. J. Genet. vol. 63, pp. 343-357.
Oxender et al. (1980) Proc. Natl. Acad. Sci. USA vol. 77, pp. 1412-1416.
Hoechst Schering AgrEvo GmbH
Nelson Amy J.
Robinson Douglas W.
LandOfFree
DNA sequences for an amino acid transporter, plasmids, bacteria, does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with DNA sequences for an amino acid transporter, plasmids, bacteria,, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sequences for an amino acid transporter, plasmids, bacteria, will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1783362