Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1988-12-14
1992-04-28
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4351723, 4352402, 43525233, 4352523, 435255, 4353201, 536 27, C12N 960, C12N 1500, C07H 1512
Patent
active
051089190
ABSTRACT:
A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.
REFERENCES:
patent: 4757013 (1988-07-01), Inouye et al.
patent: 4771002 (1988-09-01), Gelvin
Day et al. (1987), "Molecular Cloning of cDNA coding for Human PGP 9.5", FEB Letters, vol. 210, pp. 157-160.
Matsui et al. (1982), "Isopeptidase", PNAS, vol. 79, pp. 1535-1539.
Okayama et al. (1983), "A cDNA Cloning Vector", MCB, vol. 3, pp. 280-289.
Wood (1987), "Gene Cloning Based on Long Oligonucleotide Probes", in Methods in Enzymology, vol. 1542 (Academic Press, N.Y.), pp. 443-447.
Kanda et al. (1986), "Substrate Recognition of Isopeptidase", BBA, vol. 870, 64-75.
Rechsteiner, "Ubiquitin-Mediated Pathways for Intracellular Proteolysis", Ann. Rev. Cell Biol., 3: 1-30 (1987).
Rechsteiner, M., Ubiquitin, Chapters 2, 5, and 6 (N.Y.: Plenium Press, 1988).
Ozkaynak et al., "The Yeast Ubiquitin Genes: a Family of Natural Gene Fusions", pp. 1429-1439, The EMBO Journal, 6: (1987).
Lund et al., "Nucleotide Sequence Analysis of a cDNA Encoding Human Ubiquitin Reveals that Ubiquitin is Synthesized as a Precursor", J. Biol. Chem., vol. 260, pp. 7609-7613 (1985).
Barr et al., "Production of Recombinant DNA-Derived Pharmaceuticals in the Yeast Saccharomyces Cerevisiae", Abst. 34, Amer. Chem. Soc., Sep. 25-30, 1988.
Bachmair et al., Science, 234: 179-186 (1986).
Butt et al., PNAS U.S.A., 86: 2540-2544 (1989).
Hough et al., J. Biol. Chem., 261 (5): 2400-2406 (1986).
Mayer & Wilkinson, Biochemistry, 28: 166-172 (1989).
Duerksen-Hughes et al., Biochemistry, 28: 8530-8536 (1989).
Wilkinson et al., Science, 246: 670-673 (1989).
Pickart and Rose, "Mechanism of Ubiquitin Carboxyl-Terminal Hydrolase", pp. 10210-10217, vol. 261 (Aug. 1986) The Journal of Biological Chemistry.
Pickart and Rose, J. Biol. Chem., 260 (13): 7903-7910 (1985).
Liu Chung-Cheng
Miller Harvey I.
Genentech Inc.
Hasak Janet E.
Lebovitz Richard M.
Schwartz Richard A.
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