DNA sequences encoding yeast ubiquitin hydrolase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4351723, 4352402, 43525233, 4352523, 435255, 4353201, 536 27, C12N 960, C12N 1500, C07H 1512

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051089190

ABSTRACT:
A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

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