DNA sequences encoding the plant toxin gelonin

Details DNA sequences encoding the plant toxin gelonin DNA sequences encoding the plant toxin gelonin

1994-10-21

1997-04-29

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 691, 435243, 4353201, 435348, 435325, 435354, 435362, 435366, 435419, 43525233, 43525234, 43525235, 43525231, 4352542, 536 236, C12P 1934, C12P 2102, C12N 1563, C07H 2104

Patent

active

056248270

ABSTRACT:
The present invention provides the nucleotide sequence for a synthetic gene for the plant toxin gelonin and a process for producing, cloning and expressing this synthetic gene. The DNA sequence for a synthetic gene for gelonin as shown in sequence ID NO. 1. The present invention also provides expression vectors containing the DNA sequences for gelonin and cells transformed with these vectors. In addition, an immunotoxin comprising an antibody of conjugated to the protein gelonin.

REFERENCES:
Shatzman, et al., Expression, Identification, and Characterization of Recombinant Gene Products in Escherichia coli, Methods in Enzymology, vol. 152, 1987, pp. 661-673.
Brinkman et al., B3(Fv)-PE38KDEL, a single-chain immunotoxin that causes complete regression of a human carcinoma in mice, Proc. Natl. Acad. Sci. USA, vol. 88, pp. 8616-8620, Oct. 1991.
Montanaro et al., A Metalloproteinase Associated with Gelonin, A Ribosome Inactivating Protein, Ital. J. Biochem., 1984, pp. 1-10.
Hostomsk y et al. (1987) Nucleic Acids Res. 15:4849-4856.
Aota, S. et al. (1987) Nucleic Acids Res. 165:r315-r391.
Chaudhary, V.K. et al. (1989) Nature 339: 394-397.
Montecucchi et al (1989) Int. J. Peptide Protein Res. 33: 263-267.
Young et al (1983) Proc. Nat. Acad. Sci, USA, 80: 1194-1198.
Falasco et al (1982) Biochem J. 207:505-509.
Stirpe F, et al. (1980) J. Biol. Chem 255:6947-6953.
Wosnick, M.A. et al (1987) Gene 60:115-127.
Berger, S.L. et al (1987) Methods in Enzymology 152 "Guide to Molecular Cloning Techniques" New York: Academic Press.
Sambrook, J et al. (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Press.

Affiliated with

Also associated with

Rating

DNA sequences encoding the plant toxin gelonin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA sequences encoding the plant toxin gelonin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sequences encoding the plant toxin gelonin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-705032