Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-09-02
2000-05-16
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
800278, 800282, 800287, 536 236, 536 241, 4352523, 43525233, 4353201, 435419, 435421, 4354301, 435468, C12N 1529, C12N 504, C12N 1582, A01H 500, A01H 510
Patent
active
060639881
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a novel DNA sequence and its use for transforming vectors, host organisms and plants and for producing novel plants which are male-sterile and which exhibit an altered flower colour.
2. Description of Related Art
Male-sterile plants play an important role in plant breeding, in particular in hybrid breeding. A variety of methods for producing male-sterile plants have already been disclosed, which methods involve, for example, eliciting cell damage specifically, for example in the anthers, interfering in mitochondrial functions, using antisense DNA to create opportunities for chemicals to exert a sterilizing effect or inhibiting chalcone synthesis (cf. WO 90/08830, WO 90/08831, WO 89/10396, EP-A-0 329 308 and EP-A-0 335 451). However, the methods which have hitherto been available for producing male-sterile plants do not, in many cases, lead to completely satisfactory results. In addition to this, plants are frequently obtained which exhibit a considerably increased susceptibility towards fungal pathogens, making it substantially more difficult to handle them in practice. There is, therefore, a great need for other methods of producing male-sterile plants which do not suffer from these disadvantages.
The production of plants which exhibit an altered flower colour is of particular interest for ornamental plant breeding, so that there is considerable interest in new methods in this field as well.
SUMMARY OF THE INVENTION
The novel DNA sequence, which is termed DNA sequence I below, has now been found, which sequence consists of the following components, which are sequentially ordered in the 5'-3' direction: strongly active in plants and/or which is anther-specific or tapetum-specific, and which is, where appropriate, located downstream of an amplifying element (enhancer); which still exhibit the features which are essential for implementing the invention.
It has furthermore been found that plants which harbour DNA sequence I in their genome are, surprisingly, male-sterile and, in addition to this, exhibit a flower colour which is altered as compared with the corresponding plants which do not contain the DNA sequence I.
These novel plants additionally possess an increased resistance towards microbial plant pathogens, in particular towards phytopathogenic fungi. In many cases, the altered flower colour makes it easier to identify the male-sterile plants readily in a mixed population, something which can be of considerable practical relevance.
The present invention consequently also relates to novel plants (including parts of these plants and their replicative material, such as protoplasts, plant cells, calli, seeds, tubers or cuttings, etc.) which harbour the DNA sequence I in their genome and which are male-sterile and/or exhibit a flower colour which is altered as compared with the corresponding plants which do not harbour the DNA sequence I.
BRIEF DESCRIPTION OF THE DRAWING
FIG. 1 shows the construction of plasmid pSSVst1.
DETAILED DESCRIPTION OF THE INVENTION
Promoters which are strongly active in plants and which can be used, in accordance with the invention, as component a) of the DNA sequence I have been disclosed. The promoter of the gene of the small subunit of ribulose-1,5-bisphosphate carboxylase (rbcS) may be mentioned as an example (cf., e.g., EMBO Journal, Vol. 5, No. 9, 2063-2071 (1986)). Furthermore, plant virus promoters which are strongly active in plants may also be employed. Such promoters have been disclosed, and the CaMV 35S promoter (cf., e.g., Science 250: 959-960 (1990)) may be mentioned by way of example.
Anther-specific and/or tapetum-specific promoters may also be used as component a) of the DNA sequence I. Such promoters, which display their activity particularly strongly in the anthers or in the anther site termed the tapetum, have been disclosed. The TA29 promoter may be mentioned as an example (cf., e.g., Nature 347, 737-741 (1990)). The known anther-specific promoters, which have been
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Fischer Regina
Hain Rudiger
Bayer Aktiengesellschaft
Fox David T.
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