DNA sequences encoding phytases of ruminal microorganisms

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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536 237, 536 232, 4351723, 435196, 4352523, 43525231, 43525233, 43525423, 43525411, 435419, 435325, 800205, C12N 1531, C12N 1555, C12N 914, C12N 115, C12N 121, C12N 510, A01H 500

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059856050

ABSTRACT:
Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

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