DNA sequences encoding human TcAK1 kinase

Details DNA sequences encoding human TcAK1 kinase DNA sequences encoding human TcAK1 kinase

1996-07-09

1999-01-26

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352401, 4353201, 536 231, 536 235, 536 2431, 935 2, 935 6, 935 77, 935 78, C12Q 168, C07H 2104, C12N 1585

Patent

active

058637290

ABSTRACT:
Entry into mitosis requires the activity of the Cdc25C phosphatase which functions to activate Cdc2/Cyclin B. In asychronously growing cells, Cdc25C is stoichiometrically phosphorylated on serine 216. Levels of serine 216 phosphorylation remain constant throughout the G1-, S- and G2-phases of the cell cycle. A human kinase, denoted TcAK1 (for Twenty-five C Associated protein Kinase) that phosphorylates Cdc25C on serine 216 has been cloned and sequenced. A method is also provided for measuring levels of TcAK1 in RNA or of TcAK1 protein in cells. Phosphorylation of Cdc25C on serine 216 with TCAK1 creates a 14-3-3 recognition motif. The interaction between Cdc25C and 14-3-3 proceeds in a phosphorylation-specific manner. TcAK1 functions to mediate interaction between 14-3-3 proteins and other cellular proteins associated with oncogenesis or key signalling events.

REFERENCES:
Ogg et al. (1994), "Purification of a Serine Kinase That Associates with and Phosphorylates Human Cdc25C on Serine 216," J. Biol. Chem. 269:30461-30469.
Ford et al. (1994), "14-3-3 Protein Homologs Required for the DNA Damage Checkpoint in Fission Yeast," Science 265:533-535.
Morrison (1994), "14-3-3: Modulators of Signaling Proteins?" Science 266:56-57.
Aitken (1995), "14-3-3 Proteins on the MAP," TIBS 20:3pp.
Muslin et al. (1996), "Interaction of 14-3-3 with Signaling Proteins is Mediated by the Recognition of Phosphoserine," Cell 84:889-897.
Accession M80359.
A Usubel et al Editors of Short Protocols In Molecular Biology. John Wiley & Sons, New York (1989) pp. 75-79, 106-116, and 158-159.
Sequence Alignment of p78 and Seq. ID Nos. 1 & 2.

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