Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2004-10-15
2008-11-25
Prouty, Rebecca E. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S069100, C435S252300, C435S471000, C435S203000, C536S023200
Reexamination Certificate
active
07456003
ABSTRACT:
DNA sequences coding for enzymes capable of facilitating the synthesis of linear □-1,4 glucans in plants, fungi and microorganisms. The invention relates to DNA sequences Coding for proteins having the enzymatic activity of an amylosucrase which allows the synthesis of linear □1,4 glucans from the substrate sucrose by bacteria, fungi and plants or in cell-free systems. The invention furthermore describes plasmids and bacteria containing these DNA sequences as well as processes for the production of plants and microorganisms capable of intracellularly or extracellularly expressing a polypeptide having amylosucrase activity. The invention furthermore relates to the production of pure fructose using proteins exhibiting the enzymatic activity of amylosucrase.
REFERENCES:
Okada et al. JBC 1974, 249, 126-135.
Montalk et al. J. Bactrol. 1999,pp. 375-381.
Hizukuri et al., “Multi-branched nature of amylose and the action of debranching enzymes,”Carbohydrate Research, 94: 205-213 (1981).
Takeda et al., “Structures of branched and linear molecules of rice amylose,”Carbohydrate Research, 246: 267-272 (1993).
Buttcher Volker
Kossmann Jens
Welsh Thomas
Bayer Bioscience GmbH
Haley Jr. James F.
Meah Younus
Prouty Rebecca E.
Ropes & Gray LLP
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