DNA sequences and plasmids for the preparation of plants with ch

Details DNA sequences and plasmids for the preparation of plants with ch DNA sequences and plasmids for the preparation of plants with ch

1997-01-03

1999-11-02

Smith, Lynette R. F.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Treating animal or plant material or micro-organism

4353201, 536 236, C12N 1529, C12N 1582

Patent

active

059768690

ABSTRACT:
This invention relates to DNA sequences and plasmids containing these sequences which can be integrated into a plant genome and which modify the activity of the sucrose-phosphate-synthase (sps) of the plant. The invention relates further to derivatives of the DNA sequences of the invention that are obtained by exchange of single bases or by deletion or insertion of base sequences and which code for proteins with comparable activity to sucrose-phosphate-synthase. Methods for transforming plant cells and regenerating plants from the cells which exhibit modified sucrose-phosphate-synthase activity are also described.

REFERENCES:
Stam M, et al. "The silence of genes in transgenic plants." Ann. Bot. 79: 3-12, 1997.
Koziel MG, et al. "Optimizing expression of transgenes with an emphasis on post-transcriptional events." Plant Mol. Biol. 32: 393-405, 1996.
Smith CJS, et al. "Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes." Nature 334: 724-726, Aug. 25, 1988.
Worrell AC, et al. "Expression of a maize sucrose phosphate synthase in tomato alters leaf carbohydrate partitioning." Plant Cell 3: 1121-1130, 1991.
Salanoubat M, et al. Molecular cloning and sequencing of sucrose synthase cDNA from potato (Solanum tuberosum L.): Preliminary characterization of sucrose synthase mRNA distribution, 1987.
Carter P. "Site-directed mutagenesis." Biochem. J. 237: 1-7, 1986.

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