Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-07-29
1997-03-11
Stanton, Brian R.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 435 701, 435 711, 435243, 4353201, 435325, 435366, 435372, 4353722, 4353723, 536 241, C07H 2104, C12N 510, C12N 1511, C12P 2100
Patent
active
056100531
ABSTRACT:
A newly-characterized chromatin insulator element isolated from the DNA of a higher eukaryotic organism and contained in vector constructs is described. The insulator element of the invention comprises a DNA sequence which contains a 5' constitutive hypersensitive site whose functional activity and biochemical characterization as a pure insulator were previously unknown. A core DNA sequence having strong insulator activity is described. The insulator element, including the core sequence, have been demonstrated for the first time in mammalian cells to function to buffer or insulate an expressed gene from the activity of cis-acting regulatory elements, such as enhancers, in the surrounding chromatin or DNA.
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Chung Jay H.
Felsenfeld Gary
Stanton Brian R.
The United States of America as represented by the Department of
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