DNA sequence useful for the production of polyhydroxyalkanoates

Details DNA sequence useful for the production of polyhydroxyalkanoates DNA sequence useful for the production of polyhydroxyalkanoates

1996-08-13

1998-05-12

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

4351723, 435183, 4352523, 4352533, 4353201, 435419, 43525234, 536 232, 536 237, 800250, 800DIG9, 800DIG14, 800DIG15, 800DIG23, 800DIG26, 800DIG42, 800DIG43, 800DIG56, 800DIG58, 530350, A01H 500, C12N 120, C12N 121, C12N 514

Patent

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057508488

ABSTRACT:
A genomic fragment harboring the gene phaG was cloned by phenotypic complementation of Pseudomonas putida KT2440 mutants, defective in the polyhydroxyalkanoic acid (PHA) synthesis via de novo fatty acid biosynthesis but not affected in PHA biosynthesis via fatty acid .beta.-oxidation. The 885-bp phaG gene encodes a protein of 295 amino acids with a molecular weight of 33,876 Da. The transcriptional induction of phaG could be observed under conditions of PHA synthesis via de novo fatty acid biosynthesis whereas no induction was detected under conditions which favor PHA synthesis via fatty acid degradation by .beta.-oxidation. The phaG gene is useful for the production of PHAs in bacteria and plants.

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Ochsner et al., "Isolation and Characterization of a Regulatory Gene Affecting Rhamnolipid Biosurfactant Synthesis in Pseudomonas aeruginosa," Journal of Bacteriology 176:2044-2054 (1994).
Ochsner et al., "Autoinducer-Mediated Regulation of Rhamnolipid Biosurfactant Synthesis in Pseudomonas aeruginosa," Proc. Natl. Acad. Sci. USA 92:6424-6428 (1995).
Ochsner et al., "Isolation, Characterization, and Expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB Genes Encoding a Rhamnosyltransferase Involved in Rhamnolipid Biosurfactant Synthesis", Journal of Biological Chemistry 269:19787-19795 (1994).
Koch et al., "Hydrocarbon Assimilation and Biosurfactant Production in Pseudomonas aeruginosa Mutants," Journal of Bacteriology 173:4212-4219 (1991).
GenBank accession L012105: Quinolone sensitivity protein from P. aeruginosa (1992).

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