Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus
Patent
1986-11-20
1991-05-14
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a carotene nucleus
4353201, 4351723, 43525231, 536 27, 935 48, 935 60, 935 74, C12N 1500, C12N 1563, C12N 1575
Patent
active
050155741
ABSTRACT:
A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.
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Akaoka Akiko
Furutani Yoshio
Honjo Masaru
Kawamura Kouichi
Mita Izumi
Agency of Industrial Science and Technology
Extra-Ministerial Bureau of Ministry of International Trade and
Nolan S. L.
Schwartz Richard A.
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