DNA sequence imparting cytoplasmic male sterility, mitochondrial

Details DNA sequence imparting cytoplasmic male sterility, mitochondrial DNA sequence imparting cytoplasmic male sterility, mitochondrial

1995-06-06

1998-08-04

Houtteman, Scott W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

800DIG15, 800255, 4353171, 435410, 435419, C12N 1500

Patent

active

057895660

ABSTRACT:
Ogura sterility DNA is used to develop agronomically valuable hybrids that lack the undesirable features of plants having Ogura DNA (chlorosis at low temperature; poor fertile fertility; aberrant flower morphology) but still impart efficient male sterility (which can be easily restored). Ogura sterility is carried by a DNA sequence defined by nucleotides 928-2273 of a 2428 base sequence reproduced in the specification. When present in mitochondrial or nuclear genome of a plant it confers cytoplasmic male sterility. Also disclosed are recombinant plant nuclear and mitochondrial genomes containing a DNA sequence defined by nucleotides 928-1569 of the specified sequence (or its homologous); cytoplasm containing such a mitochondrial genome; Brassica plants or hybrids containing the sterility DNA; and, DNA probes of at least 10 bases from the 928-1569 sequences. DNA probes are used to detect male sterility and for selecting clones without the need for lengthy agronomic/rehybridization steps.

REFERENCES:
Pelletier et al. Molec. Gen. Genet. 191: 244-250 (1983).
Chetrit et al. Theor. Appl. Genet. 69: 361-366 (1985).
Vedel et al. Plant Physiol. Biochem 25: 249-257 (1987).
Makaroff et al. Jour. Biological Chem. 264: 11706-11713 (1989).
Bonhomme et al. Curr. Genet. 19: 121-127 (1991).
Makaroff Sequence Alligment with SEQ ID No: 1 (3 pages).
Reeck et al., Cell 50: 667, Aug. 28, 1987.
Lewin, Science 237: 1570, 1987.
Turpen et al., Mol. Gen. Genet. 209(2):227-233, 1987.

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