DNA sequence encoding sterol .DELTA.14 reductase

Details DNA sequence encoding sterol .DELTA.14 reductase DNA sequence encoding sterol .DELTA.14 reductase

1995-05-11

1996-04-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 691, 4351723, 435198, 4352523, 43525421, 4353201, 536 232, 935 14, 935 28, 935 69, C12N 902, C12N 1553, C12N 1581, C12N 1509

Patent

active

055124720

ABSTRACT:
A gene encoding Saccharomyces cerevisiae sterol .DELTA.14 reductase of the ergosterol biosynthetic pathway is isolated and cloned by selecting strains carrying sequences on a 2.mu. based vector for resistance to a morpholine fungicide such as fenpropimorph. Four distinct plasmid inserts which produce morpholine resistance are obtained, and one of these is characterized and sequenced. The purified and isolated DNA sequence encoding sterol .DELTA.14 reductase encodes a polypeptide exhibiting homology to the S. cerevisiae sterol C-24(28) reductase enzyme in the ergosterol biosynthetic pathway.

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