Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1995-05-15
1996-06-11
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435 691, 4352523, 4353201, 514 2, 536 232, 935 14, 935 29, 935 69, 935 73, C12N 902, C12N 1553, C12N 1581, A61K 3844
Patent
active
055254969
ABSTRACT:
A gene encoding Saccharomyces cerevisiae sterol .DELTA.14 reductase of the ergosterol biosynthetic pathway is isolated and cloned by selecting strains carrying sequences on a 2.mu. based vector for resistance to a morpholine fungicide such as fenpropimorph. Four distinct plasmid inserts which produce morpholine resistance are obtained, and one of these is characterized and sequenced. The purified and isolated DNA sequence encoding sterol .DELTA.14 reductase encodes a polypeptide exhibiting homology to the S. cerevisiae sterol C-24(28) reductase enzyme in the ergosterol biosynthetic pathway.
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Bard Martin
Hsien-fen Kuh Lai Margaret
Kirsch Donald R.
American Cyanamid Company
Krinsky Mary M.
Moore William W.
Patterson Jr. Charles L.
Webster Darryl L.
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