DNA sequence encoding endoglucanase III cellulase

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 232, 435209, C12N 942, C12N 1556

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active

054751012

ABSTRACT:
The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.

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Ward, et al., "Cloning, sequence and preliminary structural analysis of a small, high pl endoglucananse (EGIII) from Trichoderma reesei" The Tricel 93 Symposium, Jun. 2-5, 1993, Espoo, Finland, pp. 153-158 in Foundation For Biotechnical And Industrial Fermentation Research, vol. 8, edited by Suominen et al, (Nov. 1993).
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Jerry Stahlberg, Ph.D. Thesis, Uppsala University, Feb. 1993, pp. 16-18.

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