DNA-sequence encoding biosynthetic insulin precursors and proces

Details DNA-sequence encoding biosynthetic insulin precursors and proces DNA-sequence encoding biosynthetic insulin precursors and proces

1985-05-29

1990-04-10

Wiseman, Thomas G.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Insulin; related peptides

4351723, 435 694, 435320, 43525233, 435942, 435256, 536 27, 935 13, 935 69, C12N 1500, C12N 1200, C12N 120, C12P 2100

Patent

active

049162128

ABSTRACT:
Human insulin precursors containing the peptide chain B(1-29)-A(1-21) of human insulin and derivatives thereof with a bridging chain connecting the carboxyl terminus of the B(1-29)-chain with the amino terminus of the A(1-21)-chain are prepared by culturing a yeast host transformed with a replicable expression vehicle capable of expressing a DNA-sequence encoding the insulin precursor. The bridging chain is preferably relatively short and contains preferably from 2 to 8 amino acid residues. The bridging chain must not contain two adjacent basic amino acid residues (Lys or Arg) and has one Lys or Arg connected to the amino terminus of the A(1-21)-chain. Human insulin is prepared from the insulin precursors by in vitro conversion.

REFERENCES:
patent: 4343898 (1982-08-01), Markussen
patent: 4431740 (1984-02-01), Bell
patent: 4440859 (1984-04-01), Rutter et al.
patent: 4615974 (1986-10-01), Kingsman et al.
Greene et al, Methods in Enzymology, vol. XLVII, 1977, pp. 170-172.

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