DNA sequence coding for enhancin polypeptide which enhances viru

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

530826, 530825, 435 691, 4352401, 4352351, 4353201, 4241991, 4242041, C07K 100, C12N 1500, C12P 2106, A61K 3912

Patent

active

057170690

ABSTRACT:
This disclosure relates to an isolated and cloned DNA from a granulovirus virus which comprises an amino acid sequence of the vital gene encoding a polypeptide isolated from occlusion bodies of certain baculoviruses and which polypeptide possesses the biological activity of enhancing baculovirus infectivity. Such proteins termed herein as "enhancins" are found within the viral occlusion body, have a disruptive effect on the insect peritrophic membrane (PM) proteins, and/or interact with the midgut epithelium in such a manner as to permit the increased adsorption, penetration and uptake of virus particles by midgut cells with a concomitant increase in host mortality. Disclosed herein is a recombinant DNA sequence which codes for the enhancin protein of the Helicoverpa armigera granulovirus virus. The DNA sequence is shown in SEQ. ID. NO.: 1 and the open reading frame is shown in SEQ. ID. NO.: 1: base pairs 271-2976. The amino acid sequence of the enhancin protein is shown in SEQ. ID. NO.: 2.

REFERENCES:
patent: 4904645 (1990-02-01), Puritch et al.
patent: 4973667 (1990-11-01), Granados
patent: 5011685 (1991-04-01), Granados
patent: 5475090 (1995-12-01), Granados et al.
Hoshimoto et al, 1991, J. Gen. Virology, v. 72 pp. 2645-2651.
Roelvink, P.W. et al, 1995, Characterization of the Helicoverpa armigera and Pseudaletia unipuncta granulovirus enhancing genes, Jnl. Of Gen. Virology, 76: 2693-2705.
Hamm, J.J., 1982, Extension of the Host Range for a Granulosis Virus from Heliothis armiger from South Africa, Environmental Entomology, vol. II, No. 1, pp. 159-160.
Derksen, A.C. et al, 1988, Alteration of a Lepidopteran Peritrophic Membrane by Baculoviruses and Enhancement of Viral Infectivity, Virology, 167:242-250.
Tanada, Y., et al, 1975, Enzyme synergistic for insect viruses, Nature, Nol. 254:328-329.
Tanada, Y, et al, 1985, A Synopsis of Studies on the Synergistic Property of an Insect Baculovirus: A Tribute to Edward A. Steinhaus, Jnl. Of Invert. Pathology, 45: 125-138.
Tanada, Y., et al, 1959, Synergism between Two Viruses of the Armyworm, Pseudalertia unipuncta (Haworth) (Lepidoptera, Noctuidae).sup.1, Jnl. Of Insect Pathology 1:215-231.
Blissard, G.W. et al, 1990, Baculovirus Diversity and Molecular Biology, Annu. Rev. Entomo., 35:125-125.
Wood, H.A. et al, 1991, Genetically Engineered Baculoviruses as Agents For Pest Control, Annu. Rev. Microbiol., 45:69-87.
Corsaro, B. Et al, Baculovirus Enhancing Proteins as Determinants of Viral Pathogenesis, Paraasites and Pathogens of Insects, vol. 2, pp. 127-145.
Miller. L.K. 1988, Baculoviruses as Gene Expression Vectors, Ann. Rev. Microbiol. 42: 177-99.
Westwood, J. Et al, 1993, Analyses of Alternative Poly (A) Signals for Use in Baculovirus Expression Vectors, Virology 195, 90-99.
Gallo, L.G. et al, 1994, In Vivo Enhancement of Baculovirus Infection by the Viral Enhancing Factor of a Granulosis Virus of the Cabbage Looper, Trichoplusis ni (Lepidopters: Noctuidae), Jnl. Of Invertebrate Pathology 58, 203-210.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

DNA sequence coding for enhancin polypeptide which enhances viru does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA sequence coding for enhancin polypeptide which enhances viru, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sequence coding for enhancin polypeptide which enhances viru will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2078249

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.