Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-07-28
1997-01-14
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
055938302
ABSTRACT:
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotide.
REFERENCES:
patent: 5424184 (1995-06-01), Santamaria et al.
Parnham et al. Diversification of HLA-A, B, C Alleles, J. of Immunology, 142: 3937-3950, 1989.
Barbosa Jose J.
Boyce-Jacino Michael T.
Faras Anthony J.
Rich Stephen S.
Santamaria Pedro
Jones W. Gary
Rees Dianne
Regents of the University of Minnesota
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