DNA sequence-based HLA class I typing method

Details DNA sequence-based HLA class I typing method DNA sequence-based HLA class I typing method

1991-05-08

1995-06-13

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 15, 435 912, 435 9121, 536 2433, 935 8, 935 17, 935 18, 935 77, 935 78, C12Q 148, C12Q 168, C12N 1511, C07H 2104

Patent

active

054241847

ABSTRACT:
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotides.

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