DNA-sequence-based diagnosis of mastitis from a milk sample

Details DNA-sequence-based diagnosis of mastitis from a milk sample DNA-sequence-based diagnosis of mastitis from a milk sample

1996-02-27

1998-12-15

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 911, 536 243, 536 2433, 536 231, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

058494880

ABSTRACT:
A rapid method for diagnosing mastitis is described. The method is based on DNA sequence identification comprising the steps of determining the presence of the following DNA sequences in a milk sample: a DNA sequence specific for somatic cells for indicating inflammation; a DNA sequence specific for a mastitis pathogen for indicating infection; and a DNA sequence specific for an antibiotic-resistance-encoding gene of a pathogen for assisting a proper drug therapy. Test kits and genus- or species-specific oligonucleotides and their use in said method are also described.

REFERENCES:
patent: 5536638 (1996-07-01), Rossau et al.
Barry, T., et al, Research, 1:51-56 (1991) by Cold Spring Harbor Laboratory Press ISSN 1054-9803/91, PCR Methods and Applications, "The 16s/23s Ribosomal Spacer Region as a Target for DNA Probes to Identify Eubacteria".
T. Lam, et al, Am. J. Vet. Res., vol. 57, No. 1, Jan .1996, pp. 39-42, "Epidemiological characteristics of bovine clinical mastitis caused by Staphylococcus aureus and Escherichia coli studied by DNA fingerprinting".
W. Zneifel, et al, Zentralblatt Fur Hygiene Und Umweltmedizin, vol. 192, No. 6, Mar. 1992, pp. 544-553, XP000675359, "Adaption of Two Commercially Available DNA Probes for the Detection of E. coli and Staphylococcus aureus to Selected Fields of Dairy Hygiene--An Exemplary Study".
B.E. Gillespie, et al, Journal of Dairy Science, vol. 78, No. suppl. 1, 25 Jun. 1995, p. 172 XP000674569, "Polymerase chain reaction-based DNA fingerprinting method for identification of Streptococcus species isolated from bovine milk", See Abstract p. 63.
R.W. Bentley, et al, Journal of clinical Microbiology, vol. 31, No. 1, Jan. 1993, pp. 57-60, XP 000675362, "Development and Use of Species-Specific Oligonucleotide Probes for Differentiation of Streptococcus uberis and Streptococcus parauberis".
Database WPI, Week 9347, Derwent Publications Ltd., London, GB; AN 93-370751, XP002032801, & FI 9 201 046 A (Futekno oy), 12 Sep. 1993, see abstract.

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