DNA sequence, and recombinant preparation of the grass...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

Reexamination Certificate

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C424S275100, C514S002600, C530S350000

Reexamination Certificate

active

07846448

ABSTRACT:
The present invention relates to the provision of a DNA sequence of the major grass pollen allergen Lol p 4. The invention also encompasses fragments, new combinations of partial sequences and point mutants having a hypoallergenic action. The recombinant DNA molecules and the derived polypeptides, fragments, new combinations of partial sequences and variants can be utilized for the therapy of pollen-allergic diseases. The proteins prepared by recombinant methods can be employed for in vitro and in vivo diagnosis of pollen allergies.

REFERENCES:
patent: 6759234 (2004-07-01), Gefter et al.
patent: WO 96/07428 (1996-03-01), None
Tarzi et al. ‘Pepetide immunotherapy for allergic disease.’ Expert. Opin. Biol. Ther. 3(4):617-626, 2003.
Zhou et al. ‘Regulation of levels of serum antibodies to ryegrass pollen allergen Lol pIV by an internal image anti-idiotypic monoclonal antibody.’ Immunology. 84:343-349, 1995.
Bose et al. ‘Human and murine antibodies to Rye grass pollen allergen LolpIV share a common idiotope.’ Immunology. 63:579-584, 1998.
Focke et al. Nonanaphylactic synthetic peptides derived from B cell epitopes of the major grass pollen allergen, Phl p 1, for allergy vaccination. FASEB Journal, 15:2042-2044, 2001.
Ekramoddoullah A K M, et al: “Immunochemical Characterization of a High Molecular Weight Basic Allergen of Rye Grass Lolium-Perenne Pollen” Molecular Immunology, Bd. 20, Nr. 4, 1983, p. 465-474, XP002323104; ISSN; 0161-5890.
Jaggi K et al: “Identificationof Two Distinct Allergenic Sites on Ryegrass Pollen Allergen Lol p IV” Journal of Allergy and Clinical Immunology, Bd. 83, Nr. 4, 1989, pp. 845-852, XP009045856, ISSN: 0091-6749.
Jaggi et al: “Allergenic Fragments of Ryegrass Lolium-Perenne Pollen Allergen Lol p-IV” International Archives of Allergy and Applied Immunology, Bd. 89, Nr. 4, 1989, pp. 342-348; XP009045855; ISSN: 0020-5915.
Fahlbusch et al.“Detection and Quantification of Group 4 Allergens in Grass Pollen Extracts Using Monoclonal Antibodies” Clinical and Experimental Allergy, Blackwell Scientific Publications, London, GB, Bd. 28, Nr. 7, Jul. 1998, pp. 799-807, XP002260345.
Databas EMBL; Nov. 19, 2004; “Lolium Perenne Partial LolP4 Gene for Pollen Allergen Lol P4.” XP002323105.

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