DNA sequence analysis

Details DNA sequence analysis DNA sequence analysis

2008-04-01

2008-04-01

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024500

Reexamination Certificate

active

07351532

ABSTRACT:
There is disclosed a method for determining the identity of one or more mutations or single nucleotide polymorphisms (SNPs) in a genome, comprising: a contacting a sample genome, under conditions which permit template dependant oligonucleotide ligation, with a plurality of different oligonucleotide molecules which comprise (i) a first set of oligonucleotides each comprising a sequence of nucleotides that is complementary to a region on said genome that includes a known SNP site and which oligonucleotides are complementary to said region other than at a base at or near the 5′ end of said oligonucleotides that is to be tested for complementarity to a base at the SNP site, each of said oligonucleotides comprising a unique label to identify both the base to be tested and the position of the SNP to be scored, (ii) a second set of oligonucleotides each comprising a sequence of nucleotides complementary to a region on said target genome for hybridisation with said target genome adjacent the 5′ end of an oligonucleotide of said first oligonucleotide set, and a surface capture moiety, a phosphate moiety being located at any of either the 5′ end of said first set of oligonucleotides or the 3′ end of said second set of oligonucleotides, any resulting ligated oligonucleotide being immobilised on a solid support via the surface capture moiety, b. analysing said solid support for the identity of one or more of said unique labels and comparing the defined bases in any of said immobilised oligonucleotides to those of the reference one or more SNPs.

REFERENCES:
patent: 4988617 (1991-01-01), Landegren et al.
patent: 5476930 (1995-12-01), Letsinger et al.
patent: 5846719 (1998-12-01), Brenner et al.
patent: 5952174 (1999-09-01), Nikiforov et al.
patent: 6787308 (2004-09-01), Balasubramanian et al.
patent: 2005/0287526 (2005-12-01), Landegren et al.
patent: 0 195277 (1986-09-01), None
patent: WO 95/21271 (1995-08-01), None
patent: WO 97/08344 (1997-03-01), None
patent: WO 01/57248 (2001-08-01), None
patent: WO 02/053778 (2002-07-01), None
patent: WO 02/061127 (2002-08-01), None
Xu et al., High sequence fidelity in a non-enzymatic DNA autoligation reaction, Nucleic Acids Research, 27:875-881 (1999).

Affiliated with

Also associated with

Rating

DNA sequence analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DNA sequence analysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DNA sequence analysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2799500